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Summary Statistics

Calculate per-file channel statistics and add the results to the sample table.

7. Summary Statistics

Calculate Statistics opens a window where you can pick any loaded channel and one or more statistics — Mean, Std Dev, Median, Min, Max, or All — computed per file across every loaded sample (not just checked rows).

Calculate Statistics window with GFP/FITC-A selected and Mean, Std Dev, and Median checked. — Select the channel first, then choose the statistics to calculate. Mean and Std Dev are checked by default; Median, Min, Max, and All are optional.

Click Calculate to run the selected statistics. A progress bar appears in the Calculate Statistics window while PhaseFinder loads any needed channel data; files already loaded for plotting reuse their cached data.

Metadata table after statistics have been calculated, showing grouped GFP/FITC-A Summary Statistics columns for Mean, Std Dev, and Median. — Statistics are inserted into the metadata table as a grouped column set, with one value per loaded FCS file.

Results are added to the metadata table as grouped columns, such as GFP/FITC-A Summary Statistics spanning Mean, Std Dev, Median, and any other metrics you chose. These columns are included when you export the metadata table.

If you reopen the Calculate Statistics window for a channel that already has statistics, those already-computed metrics are disabled so you do not recompute the same columns. The All checkbox only selects metrics that are still available for that channel.

Statistics are calculated automatically on new FCS file loads

Once you compute a statistic for a channel, PhaseFinder remembers that channel/statistic combination for the rest of the session. Any FCS file you load afterward gets the same statistics calculated automatically and filled into the new row, with no need to reopen the Calculate Statistics window.

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